Chicken egg yolk antibodies (IgY) as an alternative to mammalian antibodies
نویسندگان
چکیده
منابع مشابه
Egg Yolk Antibodies (IgY) in Routine Diagnostic Work.
According to Linscott"s Directory of Immunological and Biological Reagents (1994/95) the commercial availability of egg-yolk antibodies (IgY) is extremely low. For preparation, cleaning and detection of IgY it would be of advantage to have a "Protein Y" available analogous to protein A and protein G for mammalian antibodies. Until now, the search for "Protein Y" was unsuccessful. IgY has been u...
متن کاملIgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation
Hens can be immunized by means of i.m. vaccination (Musculus pectoralis, left and right, injection volume 0.5-1.0 ml) or by means of Gene-Gun plasmid-immunization. Dependent on the immunogenicity of the antigen, high antibody-titres (up to 1:100,000 - 1:1,000,000) can be achieved after only one or 3 - 4 boost immunizations. Normally, a hen lays eggs continuously for about 72 weeks, thereafter t...
متن کاملGENERATION AND PHYSICOCHEMICAL CHARACTERIZATION OF CHICKEN EGG YOLK ANTIBODIES (IgY) AGAINST Salmonella typhimurium
Salmonellosis is one of the most important bacterial diseases with economic concern to all phases of the poultry. The study was aimed to generate chicken IgY against Salmonella typhimurium and its physicochemical characterization to unbolt the obstacles for the usage of IgY as therapeutic agent in Poultry. Salmonella typhimurium whole cell antigen was prepared and immunized in white leghorn chi...
متن کاملGeneration of Egg Yolk Antibodies in Chicken ( IgY ) Against Influenza M 2 ( M 2 e ) Protein
Chicken egg yolk immunoglobulins (IgY) from immunized hens have considerable advantages for the production of polyclonal antibodies. It reacts with more epitopes and avoids the interference in immunological assays. Regarding the IgY aptitudes, this study was conducted to produce chicken IgY specific for influenza M2 (M2e) protein. Fusion construct harboring C-terminal of bovine heat shock prote...
متن کاملPurification and Characterization of Chicken Egg Yolk Antibodies (IgY) Against Mastitis Causing Klebsiella pneumoniae
White leghorn hens were immunized intramuscularly with formalin killed Klebsiella pneumoniae cells to generate Klebsiella pneumoniae antibodies (IgY) in egg yolk. Booster injections of increasing concentrations of antigen were given to raise the antibody level in egg yolk. Amount of protein varied from 0.87 7.4mg/ml in egg yolk throughout the immunization period. The antibodies were partially p...
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ژورنال
عنوان ژورنال: Indian Journal of Science and Technology
سال: 2010
ISSN: 0974-6846,0974-5645
DOI: 10.17485/ijst/2010/v3i4.24